Tutorials

• Single-cell RNA-seq Data Input Preparation from a Seurat Object
• Deconvolution and Decomposition of Mouse Cortex Visium Data using Spotiphy
  • Contents
  • Environment Setup: Package Installation and Loading
  • Data Preparation: Loading and Processing
  • Deconvolution: Marker Gene Selection and scRNA Reference Construction
  • Deconvolution: Cellular Proportion Estimation
  • Deconvolution: Cell Number Estimation Using Segmentation
  • Decomposition: Expression Breakdown of Each Spot to Single-cell Resolution
  • Imputation: Creation of Pseudo Single-cell resolution Image
• Conversion of Spotiphy-Derived iscRNA Data to Seurat or SparseEset Object for Downstream Analysis
• Decomposition Analysis
  • Environment Setup: Package Loading
  • Data Loading